creative-biolabs

With the rapid development of molecular biology and biotechnology, people's understanding of cancer has reached the molecular level, and began to try to prepare tumor vaccines and apply them to cancer treatment by means of genetic engineering according to the theory of tumor immunity. The design principle of cancer vaccine is to prepare cancer vaccine around how to enhance the immunogenicity of tumors and the body's anti-tumor immune response, and how to break the immune tolerance of tumors and the cgamp . An ideal cancer vaccine can not only induce active immunity and stimulate the host to produce an effective immune response, but also provide a protective long-term immune memory function for preventing recurrence of tumors. Tumor antigen polypeptide vaccines are expected to isolate, purify and amplify tumor antigens, increase the uptake and processing of tumor antigens by antigen presenting cells, and ultimately induce specific immune responses to tumor antigens. Nucleic acid...

What is the cause of thyroid in follicular adenoma? Follicular adenoma thyroid are the most common benign tumors, namely   benign follicular adenomas . Most occur in women under the age of 40. Because thyroid tumor can cause hyperthyroidism, and there is a certain malignant possibility, it should be removed as soon as possible. Patients often have a long history of thyroid nodules, early asymptomatic or only mild palpitation, weight loss, fatigue, with the development of the disease, patients with varying degrees of thyroid poisoning symptoms, most patients with symptoms of hyperthyroidism, Individual hyperthyroidism can occur. Most benign   thyroid tumors  except the functional autonomic thyroid tumor see isolated nodules of the thyroid gland, and a few are multiple nodules. The course of the disease is slow, and there can be no clinical symptoms in the clinic. Most of them are in the months to years or even longer. Because of slight discomfort or the mass is more t...

Pemphigus is an autoimmune disease, including mucous membrane pemphigoid  (MMP), bullous pemphigoid (BP), and pemphigoid gestationis (PG; Pregnancy herpes gestationis, HG).   PG occurs during pregnancy or the postpartum period. It has prodromal symptoms of fatigue, nausea, headache and severe itching. The skin lesions are mostly erythema, blisters and wheal, distributed in the abdomen, limbs and buttocks, umbilical cord and other parts. Histopathology Epidermis blister, superficial eosinophilic and neutrophil infiltration, direct immunofluorescence (DIF) showed anti-basement membrane antibody IgA, complement C3 linear deposition. PG often develops rapidly after delivery and then usually resolves spontaneously within 3 months. Recurrence is common, and the disease can be aggravated by the next menstrual period or oral contraceptives. PG can be diagnosed by biopsy, immunofluorescence, and clinical procedures. MMP and BP are the more common types of pemphigus. The clinical mani...

Phage antibody library The construction principle of phage antibody library Phage antibody library  is a successful example of phage display on genetically engineered antibody applications. The use of PCR to obtain antibody gene fragments is the key to phage antibody library. At present, common antibody genes are derived from hybridoma cells, in vitro immune cells, sensitized or non-sensitized B lymphocytes, etc., and total RNA amplification is performed from the above cells to obtain the variable regions of light chain and heavy chain of a full set of antibodies. After the gene of variable region is cloned into the phage genome, the antibody gene encoding peptide and the phage coat protein are fused, and it can be obtained as a single-chain variable region fragment (scFv) or as an antigen-binding fragment (Fab). Phage antibody repertoires on the surface of phage. Specific antibodies can be screened from the phage antibody library by adsorption elution enrichment. In additi...

In 2017, two CAR-Ts were approved by FDA, which became a breakthrough in cellular immunotherapy. That was the time when many patients began to pay attention to such magical T cell therapy. Recently, we learned that the authoritative doctor of lung cancer at the MD Anderson Cancer Center, the best cancer specialist hospital in the United States, has begun to use T cell therapy clinical trials as a treatment option for patients with advanced lung cancer . Although the United States has a high degree of acceptance for clinical trials, lung cancer patients generally participate in targeted, immune or combination therapy trials. According to the registration on Clinical Trial.gov, there are more than 500 relevant clinical trials in the world for CAR-T, but most of them are for hematological malignancies, and solid tumor research accounts for only about 1/4. In recent years, thanks to the rapid development of genetic modification technology and the relatively mature resp...